Project

Part:BBa_K3523007:Design

Designed by: Kong Yangyang   Group: iGEM20_Xiamen_city   (2020-10-18)


J23116-rbs-SOD-rbs-katA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2121
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1993
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To meet the type IIS standard, we made a synonymous mutation of the Glu56Glu in the SOD coding sequence to remove the SapI restriction site, the Ser283Ser in the katA sequence to remove the BsaI restriction site.


Source

The sequence of SOD comes from GenBank: BAA02655.1. Catalase (EC:1.11.1.6)


References